| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | SEARCH RESULT |
|
|
| |||||||
| |||||||||
* Department of Microbiology and Molecular Genetics,
Harvard Medical School, Boston, MA 02115; and Department of Immunology, Corixa
Corporation, Seattle, WA 98104
During its developmental cycle, the intracellular bacterial
pathogen Chlamydia trachomatis remains confined within a
protective vacuole known as an inclusion. Nevertheless,
CD8+ T cells that recognize Chlamydia Ags in the
context of MHC class I molecules are primed during infection. MHC
class I-restricted presentation of these Ags suggests that these
proteins or domains from them have access to the host cell cytoplasm.
Chlamydia products with access to the host cell cytoplasm
define a subset of molecules uniquely positioned to interface with
the intracellular environment during the pathogen’s developmental
cycle. In addition to their use as candidate Ags for stimulating
CD8+ T cells, these proteins represent novel candidates
for therapeutic intervention of infection. In this study, we use
C. trachomatis-specific murine T cells and an
expression-cloning strategy to show that CT442 from Chlamydia
is targeted by CD8+ T cells. CT442, also known as CrpA, is
a 15-kDa protein of undefined function that has previously been shown
to be associated with the Chlamydia inclusion membrane. We
show that: 1) CD8+ T cells specific for an
H-2Db-restricted epitope from CrpA are elicited at a significant
level (
4% of splenic
CD8+ T cells) in mice in response to infection; 2) the
response to this epitope correlates with clearance of the organism
from infected mice; and 3) immunization with recombinant vaccinia
virus expressing CrpA elicits partial protective immunity to
subsequent i.v. challenge with C. trachomatis.
This article has been cited by other articles: (Search Google Scholar for Other Citing Articles)
|
|
 |
|
  N. R. Roan and M. N. Starnbach Antigen-Specific CD8+ T Cells Respond to Chlamydia trachomatis in the Genital Mucosa J. Immunol., December 1, 2006; 177(11): 7974 - 7979. [Abstract] [Full Text] [PDF] |
|
|
| |||||
|
|
|
|
W. P. Loomis and M. N. Starnbach Chlamydia trachomatis Infection Alters the Development of Memory CD8+ T Cells J. Immunol., September 15, 2006; 177(6): 4021 - 4027. [Abstract] [Full Text] [PDF] |
|
|
| |||||
|
|
.gif) |
|
 N. R. Roan, T. M. Gierahn, D. E. Higgins, and M. N. Starnbach Monitoring the T cell response to genital tract infection PNAS, August 8, 2006; 103(32): 12069 - 12074. [Abstract] [Full Text] [PDF] |
|
|
| |||||
|
|
.gif) |
|
 C. Chen, D. Chen, J. Sharma, W. Cheng, Y. Zhong, K. Liu, J. Jensen, R. Shain, B. Arulanandam, and G. Zhong The Hypothetical Protein CT813 Is Localized in the Chlamydia trachomatis Inclusion Membrane and Is Immunogenic in Women Urogenitally Infected with C. trachomatis. Infect. Immun., August 1, 2006; 74(8): 4826 - 4840. [Abstract] [Full Text] [PDF] |
|
|
| |||||
|
|
|
|
R. J. Suchland, D. D. Rockey, S. K. Weeks, D. T. Alzhanov, and W. E. Stamm Development of Secondary Inclusions in Cells Infected by Chlamydia trachomatis Infect. Immun., July 1, 2005; 73(7): 3954 - 3962. [Abstract] [Full Text] [PDF] |
|
|
| |||||
|
|
|
|
I. Pinchuk, B. C. Starcher, B. Livingston, A. Tvninnereim, S. Wu, E. Appella, J. Sidney, A. Sette, and B. Wizel A CD8+ T Cell Heptaepitope Minigene Vaccine Induces Protective Immunity against Chlamydia pneumoniae J. Immunol., May 1, 2005; 174(9): 5729 - 5739. [Abstract] [Full Text] [PDF] |
|
|
| |||||
|
|
|
|
A. L. Gervassi, K. H. Grabstein, P. Probst, B. Hess, M. R. Alderson, and S. P. Fling Human CD8+ T Cells Recognize the 60-kDa Cysteine-Rich Outer Membrane Protein from Chlamydia trachomatis J. Immunol., December 1, 2004; 173(11): 6905 - 6913. [Abstract] [Full Text] [PDF] |
|
|
| |||||
|
|
|
|
M. K. Matyszak and J. S. H. Gaston Chlamydia trachomatis-Specific Human CD8+ T Cells Show Two Patterns of Antigen Recognition Infect. Immun., August 1, 2004; 72(8): 4357 - 4367. [Abstract] [Full Text] [PDF] |
|
|
| |||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | SEARCH RESULT |
