|
|
|
What is RSS?
Published Online: 29 Aug 2007
Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.
|
|
|
Research ArticleFast Fmoc synthesis of hAmylin1-37 with pseudoproline assisted on-resin disulfide formation | | Karen Page, Christina A. Hood, Hirendra Patel, German Fuentes, Mahendra Menakuru, Jae H. Park * | Department of Chemistry, Protein Technologies Inc., Tucson, Arizona, 4675 South Coach Drive, Tucson, Arizona, 85714, USA
| | email: Jae H. Park (info@peptideinstruments.com) |
*Correspondence to Jae H. Park, Department of Chemistry, Protein Technologies Inc., Tucson, Arizona, 4675 South Coach Drive, Tucson, Arizona, 85714, USA | fast Fmoc SPPS • human amylin1-37 • pseudoproline dipeptide • disulfide bridge |
| Human amylin (1-37) and the (1-13) fragment were synthesized with and without pseudoproline dipeptides. Thallium (III) trifluoroacetate, a mild oxidant, was used to cyclize the peptides by forming a disulfide bridge from C2 to C7. On the basis of our model studies, incorporation of a pseudoproline dipeptide decreases the amount of time necessary for the crude linear amylin (1-13) to cyclize on the resin. Without pseudoproline dipeptides, the 1-37 crude linear amylin was not pure enough to undergo the cyclization reaction. Following the cyclization studies, the synthesis time of the linear human amylin (1-37) was systematically reduced from 58 h to 8.5 h by shortening the reaction times. Cyclization and cleavage times were also reduced to 1.5 h. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd. |
Received: 8 May 2007; Revised: 15 June 2007; Accepted: 24 June 2007 10.1002/psc.909 About DOI
|
|
Related Articles
- Find other articles like this in Wiley InterScience
- Find articles in Wiley InterScience written by any of the authors
Wiley InterScience is a member of CrossRef.
|
 Need a reprint?
Paper or electronic reprints are available for all content published on Wiley InterScience. Inquiries can be submitted online.
Find out more about reprints
|
 
 | Current Protocols in Protein Science | A comprehensive collection of validated protocols for the experimental investigation of protein structure and function Now available via individual online subscription!
Go to Current Protocols
in Protein Science |
|
|
|
 | Encyclopedia of Life Sciences | Discover the leading resource in the life sciences. Full text available on a subscription basis or by single article Pay-Per-View. Free Sample Articles
now online: - Bioinformatics
- Chromosome Structure
- DNA cloning
- Human Gene Therapy
- Microorganisms: Applications in Molecular Biology
- Zebrafish as an Experimental Organism
Go to Free Sample Articles |
|
|
|
|
Save Article to My Profile
