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Journal of Peptide Science

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Volume 14, Issue 1 , Pages 97 - 101

Published Online: 24 Sep 2007

Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.
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 Research Article
Fast conventional Fmoc solid-phase peptide synthesis with HCTU
Christina A. Hood, German Fuentes, Hirendra Patel, Karen Page, Mahendra Menakuru, Jae H. Park *
Department of Chemistry, Protein Technologies Inc., Tucson, Arizona, 4675 South Coach Drive, Tucson, Arizona, 85714, USA
email: Jae H. Park (info@peptideinstruments.com)

*Correspondence to Jae H. Park, Department of Chemistry, Protein Technologies Inc., Tucson, Arizona, 4675 South Coach Drive, Tucson, Arizona, 85714, USA

Keywords
HCTU • fast Fmoc SPPS • human amylin (1-37) • oxytocin • -amyloid

Abstract
1H-Benzotriazolium 1-[bis(dimethyl-amino)methylene]-5-chloro-hexafluorophosphate (1-),3-oxide (HCTU) is a nontoxic, nonirritating and noncorrosive coupling reagent. Seven biologically active peptides (GHRP-6, 65-74ACP, oxytocin, G-LHRH, C-peptide, hAmylin1-37, and -amyloid1-42) were synthesized with reaction times reduced to deprotection times of 3 min or less and coupling times of 5 min or less using HCTU as the coupling reagent. Expensive coupling reagents or special techniques were not used. Total peptide synthesis times were dramatically reduced by as much as 42.5 h (1.8 days) without reducing the crude peptide purities. It was shown that HCTU can be used as an affordable, efficient coupling reagent for fast Fmoc solid-phase peptide synthesis. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.
Received: 28 June 2007; Revised: 18 July 2007; Accepted: 23 July 2007

Digital Object Identifier (DOI)

10.1002/psc.921  About DOI

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