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2 channel benchtop peptide synthesizer
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Applications
| Application Notes |
| AN-0001: On-Resin Disulfide Bridge Formation |
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| AN-0002: Synthesis of 65-74ACP and ABRF Using 4-Methylpiperidine as Deprotectant |
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| AN-0003: Cost-Effective Automated Phosphopeptide Synthesis on the Prelude Using Single-Shot Deliveries |
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| AN-0004: Fast Synthesis Study - Optimized Fmoc Chemistry Used to Rapidly Synthesize 32-mer C-Peptide in 8.7 Hours |
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| AN-0009: Peptoid Synthesis on the Symphony |
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| Posters |
| Fast Conventional Fmoc Synthesis of Human Beta-Amyloid(1-42) on the Symphony and Prelude |
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| Fully Automated Fast Fmoc Synthesis and On-Resin Disulfide Bridge Formation with Pseudoproline Dipeptides on the Prelude |
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| Fast Conventional Fmoc Synthesis of 65-74ACP on the Symphony and Prelude |
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| Prelude to the Future of Peptide Synthesis...Now! |
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| Symphony References |
| FlhB Regulates Ordered Export of Flagellar Components via Autocleavage Mechanism, Hedda U. Ferris, et al., J. Biol. Chem., Vol. 280, Issue 50, 41236-41242, December 16, 2005. |
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| Specific degradation of H. pylori urease by a catalytic antibody light chain, Emi Hifumi, Kenji Hatiuchi, Takuro Okuda, Akira Nishizono, Yoshiko Okamura, and Taizo Uda, FEBS Journal 272 (2005) doi:10.1111/j.1742-4658.2005.04869.x 4497-4505 © 2005 |
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| Cross-Reactivity of T Lymphocytes Recognizing a Human Cytotoxic T-Lymphocyte Epitope within BK and JC Virus VP1 Polypeptides, Ludmila Krymskaya, et al., Journal of Virology, September 2005, p. 11170-11178, Vol. 79, No. 17. |
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| Contribution of the Carboxyl Terminus of the VPAC1 Receptor to Agonist-induced Receptor Phosphorylation, Internalization, and Recycling, Christelle Langlet, et al. J. Biol. Chem., Vol. 280, Issue 30, 28034-28043, July 29, 2005. |
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| Priming in the Presence of IL-10 Results in Direct Enhancement of CD8+ T Cell Primary Responses and Inhibition of Secondary Responses, Silvia S. Kang and Paul M. Allen, The Journal of Immunology, 2005, 174: 5382-5389. |
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| Sensitivity of Undifferentiated, High-TCR Density CD8+ Cells to Methylene Groups Appended to Tumor Antigen Determines Their Differentiation or Death, Kouichiro Kawano, et al., Cancer Research 65, 2930-2937, April 1, 2005. |
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| Availability of short amino acid sequences in proteins, Joji M. Otaki, Shunsuke Ienaka, Tomonori Gotoh, and Haruhiko Yamamoto, Protein Science (2005), 14:617-625. |
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| Ghrelin Is Produced by the Human Erythroleukemic HEL Cell Line and Involved in an Autocrine Pathway Leading to Cell Proliferation, Carine De Vriese, et al., Endocrinology, Mar 2005; Vol. 146, No. 3 1514-1522. |
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| Identification of Murine Poxvirus-Specific CD8+ CTL Epitopes with Distinct Functional Profiles, Anuja Mathew, et al., The Journal of Immunology, Feb 2005; 174: 2212-2219. |
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| Interaction between Phosphatidylserine and the Phosphatidylserine Receptor Inhibits Immune Responses In Vivo, Peter R. Hoffmann, et al., The Journal of Immunology, Feb 2005; 174: 1393-1404. |
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| Integrin Fibronectin Receptors in Matrix Metalloproteinase-1-Dependent Invasion by Breast Cancer and Mammary Epithelial Cells, Yifeng Jia, et al., Cancer Research 64, 8674-8681, December 1, 2004. |
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| Human CD8+ T Cells Recognize the 60-kDa Cysteine-Rich Outer Membrane Protein from Chlamydia trachomatis, Ana L. Gervassi, et al., The Journal of Immunology, Dec 2004; 173: 6905-6913. |
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| Ghrelin Degradation by Serum and Tissue Homogenates: Identification of the Cleavage Sites, Carine De Vriese, et al., Endocrinology, Nov 2004; Vol. 145, No. 11 4997-5005. |
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| The C-terminal Tail of Presenilin Regulates Omi/HtrA2 Protease Activity, Sanjeev Gupta, et al., J. Biol. Chem., Vol. 279, Issue 44, 45844-45854, October 29, 2004. |
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| Interaction of TRAF6 with MAST205 Regulates NF- B Activation and MAST205 Stability, Huabao Xiong, Hongxing Li, Yibang Chen, Jie Zhao, and Jay C. Unkeless, J. Biol. Chem., Vol. 279, Issue 42, 43675-43683, October 15, 2004. |
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| Diazepam-binding inhibitor-related protein 1: a candidate autoantigen in acquired aplastic anemia patients harboring a minor population of paroxysmal nocturnal hemoglobinuria-type cells, Xingmin Feng, et al., Blood, 15 Oct. 2004(104) No. 8, pp. 2425-2431. |
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| Activation of naïve CD4 T cells by anti-CD3 reveals an important role for Fyn in Lck-mediated signaling, Katsuji Sugie, Myung-Shin Jeon, and Howard M. Grey, PNAS, Oct 2004; 101: 14859 - 14864. |
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| Diabetes Incidence Is Unaltered in Glutamate Decarboxylase 65-Specific TCR Retrogenic Nonobese Diabetic Mice: Generation by Retroviral-Mediated Stem Cell Gene Transfer, Paula Y. Arnold, et al., The Journal of Immunology, Sep 2004;173: 3103-3111. |
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| Functional Coupling of Chromogranin with the Inositol 1,4,5-Trisphosphate Receptor Shapes Calcium Signaling, Chi-Un Choe, Kenneth D. Harrison, Wayne Grant, and Barbara E. Ehrlich, J. Biol. Chem., Vol. 279, Issue 34, 35551-35556, August 20, 2004. |
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| Absence of Immunodominant Anti-Gag p17 (SL9) Responses among Gag CTL-Positive, HIV-Uninfected Vaccine Recipients Expressing the HLA-A*0201 Allele, Guido Ferrari, et al., The Journal of Immunology, Aug 2004;173: 2126-2133. |
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| An Inclusion Membrane Protein from Chlamydia trachomatis Enters the MHC Class I Pathway and Stimulates a CD8+ T Cell Response, Michael N. Starnbach, et al., The Journal of Immunology, Nov 2003; 171: 4742-4749. |
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| Synthetic Peptides That Exert Antimicrobial Activities in Whole Blood and Blood-Derived Matrices, Michael R. Yeaman, Kimberly D. Gank, Arnold S. Bayer, and Eric P. Brass, Antimicrobial Agents and Chemotherapy, December 2002, p. 3883-3891, Vol. 46, No. 12. |
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| Monocyte-Fibronectin Interactions, Via 5 1 Integrin, Induce Expression of CXC Chemokine-Dependent Angiogenic Activity, Eric S. White, Donna L. Livant, Sonja Markwart, and Douglas A. Arenberg, The Journal of Immunology, 2001, 167: 5362-5366. |
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| Strategies for the synthesis of labeled peptides, L Bibbs, NP Ambulos, SA Kates, A Khatri, KF Medzihradszky, G Osapay, and ST Weintraub, Journal of Biomolecular Techniques, Dec. 2000 Vol 11, Issue 4, 155-165. |
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| Impaired Anti-Viral T Cell Responses Due to Expression of the LY49A Inhibitory Receptor, Allan J. Zajac, et al., The Journal of Immunology, Nov. 1999, 163: 5526-5534. |
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| Ca2+-regulated Dynamic Compartmentalization of Calmodulin in Living Smooth Muscle Cells, Katherine Luby-Phelps, Masatoshi Hori, John M. Phelps, and Doug Won, J. Biol. Chem., Sep 1995; 270: 21532. |
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| The Alloreactive T Cell Response Against the Class Ib Molecule H2-M3 Is Specific for High Affinity Peptides, Vikram M. Dabhi, Rolf Hovik, Luc Van Kaer, and Kirsten Fischer Lindahl, The Journal of Immunology, Nov. 1998, 161: 5171-5178. |
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| Peptides from conserved regions of paramyxovirus fusion (F) proteins are potent inhibitors of viral fusion, D. M. Lambert, et al., PNAS, Vol. 93, Issue 5, 2186-2191, March 5, 1996. |
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| Tyrosine Phosphorylation of the CD3- Subunit of the T Cell Antigen Receptor Mediates Enhanced Association with Phosphatidylinositol 3-Kinase in Jurkat T Cells, Isabel de Aós, et al., J. Biol. Chem., Volume 272, Number 40, Issue of October 3, 1997 pp. 2531 |
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| PTI Publications |
| Fast conventional Fmoc solid-phase peptide synthesis with HCTU, J. Pep. Sci. Vol 14(1), (Jan 2008) 97-101. |
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| Fast Fmoc synthesis of hAmylin1-37 with pseudoproline assisted on-resin disulfide formation, J. Pep. Sci. Vol 13(12), (Dec 2007) 833-838. |
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| PS3 References |
| Interaction of Calmodulin with the Serotonin 5-Hydroxytryptamine2A Receptor:A PUTATIVE REGULATOR OF G PROTEIN COUPLING AND RECEPTOR PHOSPHORYLATION BY PROTEIN KINASE C, Justin H. Turner and John R. Raymond, JBC, Vol. 280(35), 30741-30750, Sept 2, 2005. |
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| Histone Acetyltransferase Activity of p300 Is Required for Transcriptional Repression by the Promyelocytic Leukemia Zinc Finger Protein, Fabien Guidez, et al., Molecular and Cellular Biology, July 2005, p. 5552-5566, Vol. 25, No. 13. |
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| Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virusShu-Fang Cheng, Cheng-Wei Wu, Eric Assen B Kantchev, and Ding-Kwo ChangEur. J. Biochem. 271, 4725-4736 (2004). |
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| Screening a combinatorial peptide library to develop a human glandular kallikrein 2-activated prodrug as targeted therapy for prostate cancer, Samuel Janssen, et al., Mol Cancer Ther. 2004;3:1439-1450. |
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| The polyproline II conformation in short alanine peptides is noncooperative, Kang Chen, Zhigang Liu, and Neville R. Kallenbach, PNAS | October 26, 2004 | vol. 101 | no. 43 | 15352-15357. |
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| DNA-mediated assembly of weakly interacting DNA-binding protein subunits: in vitro recruitment of phage 434 repressor and yeast GCN4 DNA-binding domain, Corrado Guarnaccia, et al., Nucleic Acids Research 2004 32(17):4992-5002. |
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| The Role of Electrostatic Interactions in Calmodulin-Peptide Complex Formation, Ingemar André, Tõnu Kesvatera, Bo Jönsson, Karin S. Åkerfeldt, and Sara Linse, Biophysical Journal 87:1929-1938 (2004). |
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| Measuring the refolding of B -sheets with different turn sequences on a nanosecond time scale, Rita P.-Y. Chen, et al., PNAS | May 11, 2004 | vol. 101 | no. 19 | 7305-7310. |
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| Calmodulin Interacts with the Third Intracellular Loop of the Serotonin 5-Hydroxytryptamine1A Receptor at Two Distinct Sites: PUTATIVE ROLE IN RECEPTOR PHOSPHORYLATION BY PROTEIN KINASE C, Justin H. Turner, et al., JBC, 279(17), 17027-17037, 2004. |
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| Primary Structure and in Vitro Antibacterial Properties of the Drosophila melanogaster Attacin C Pro-domain, David Rabel, et al., J. Biol. Chem., Vol. 279, Issue 15, 14853-14859, April 9, 2004. |
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| In Vivo Targeting of Underglycosylated MUC-1 Tumor Antigen Using a Multimodal Imaging Probe, Anna Moore, Zdravka Medarova, Andreas Potthast, and Guangping Dai, Cancer Research 64, 1821-1827, March 1, 2004. |
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| Ghrelin Can Bind to a Species of High Density Lipoprotein Associated with Paraoxonase, Nicholas J. Beaumont, et al., J. Biol. Chem., Vol. 278, Issue 11, 8877-8880, March 14, 2003. |
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| In Vivo Imaging of Proteolytic Enzyme Activity Using a Novel Molecular Reporter, Ching-Hsuan Tung, Umar Mahmood, Sebastian Bredow, and Ralph Weissleder, Cancer Res., Sep 2000; 60: 4953. |
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| Structure of the GCN5 histone acetyltransferase bound to a bisubstrate inhibitor, Arienne N. Poux, Marek Cebrat, Cheol M. Kim, Philip A. Cole, and Ronen Marmorstein, PNAS, Oct 2002; 99: 14065. |
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| Crystal Structure of a Complex Formed between a Snake Venom Phospholipase A2 and a Potent Peptide Inhibitor Phe-Leu-Ser-Tyr-Lys at 1.8 Å Resolution, Vikas Chandra, et al., J. Biol. Chem., Oct 2002; 277: 41079 - 41085. |
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| RhoA Sustains Integrin IIb 3 Adhesion Contacts under High Shear, Simone M. Schoenwaelder, et al., J. Biol. Chem., Apr 2002; 277: 14738 - 14746. |
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| Optical Imaging of Matrix Metalloproteinase-2 Activity in Tumors: Feasibility Study in a Mouse Model, Christoph Bremer, et al., Radiology, Nov 2001; 221: 523. |
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| Effects of turn residues in directing the formation of the ß-sheet and in the stability of the ß-sheet, Pei-Yeh Chen, Chih-Kai Lin, Chung-Tien Lee, Howard Jan, and Sunney I. Chan, Protein Sci., Sep 2001; 10: 1794. |
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| Transcriptional Coactivator Protein p300. KINETIC CHARACTERIZATION OF ITS HISTONE ACETYLTRANSFERASE ACTIVITY, Paul R. Thompson, et al., J. Biol. Chem., Aug 2001; 276: 33721 - 33729. |
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| Kinetic Investigation of Chemokine Truncation by CD26/Dipeptidyl Peptidase IV Reveals a Striking Selectivity within the Chemokine Family, Anne-Marie Lambeir, et al., J. Biol. Chem., Aug 2001; 276: 29839 - 29845. |
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| Phosphorylation of Centrin during the Cell Cycle and Its Role in Centriole Separation Preceding Centrosome Duplication, Ward Lutz, Wilma L. Lingle, Daniel McCormick, Tammy M. Greenwood, and Jeffrey L. Salisbury, J. Biol. Chem., 2001; 276: 20774 - 20780. |
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| Truncation of Macrophage-derived Chemokine by CD26/ Dipeptidyl-Peptidase IV beyond Its Predicted Cleavage Site Affects Chemotactic Activity and CC Chemokine Receptor 4 Interaction, Paul Proost, et al., J. Biol. Chem., Feb 1999; 274: 3988. |
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| Amidative peptide processing and vascular function, Charlie D. Oldham, et al., Am J Physiol Cell Physiol, Dec 1997; 273: 1908. |
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| Reaction Versus Subsite Stereospecificity of Peptidylglycine -Monooxygenase and Peptidylamidoglycolate Lyase, the Two Enzymes Involved in Peptide Amidation, Dongsheng Ping, et al., J. Biol. Chem., Dec 1995; 270: 29250. |
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| Peptides containing the RERMS sequence of amyloid beta/A4 protein precursor bind cell surface and promote neurite extension, LW Jin, H Ninomiya, JM Roch, D Schubert, E Masliah, DA Otero, and T Saitoh, J. Neurosci., Sep 1994; 14: 5461. |
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| Heparin-binding peptides from thrombospondins 1 and 2 contain focal adhesion-labilizing activity, JE Murphy-Ullrich, S Gurusiddappa, WA Frazier, and M Hook, J. Biol. Chem., Dec 1993; 268: 26784 - 26789. |
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| Technical Notes |
| TN-0001: Prelude Cleaning Procedures |
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| TN-0006: Double Couple Expensive Amino Acids on the Prelude using Single-Shot Deliveries and Dynamic Sequence Programming |
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| TN-0008: Symphony Shut-Down Procedures |
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